Structure of Myometrium Architecture

Structure of Myometrium ArchitectureTHE STRUCTURAL AND ULTRASTRUCTURAL PARTICULARITIES OF THE MYOMETRIUM ARCHITECTUREAnt nonp beillla Chesc1, M.A.Moga1, Mariana Tilinca2, H. Matei3SummaryThe morphological aspects of the human non-pregnant and pregnant at cod clock myometrium were emphasized by the authoritative histological technique and the electronic microscopic technique. We breakd the structural changes that appear at the take aim of the myocytes, alignment and myometrial vascular structures. Both the stage of non-pregnant womb and the one of pregnant uterus at due time present structural and ultra-structural particularities at the level of the myocytes, conjugation structures and of the myometrial vascular ones. Key words myometrium, myocytes, optical microscopy, electronic microscopyThe myometrium is made up of smooth muscular fibers that are internally disposed in layers with greenback fibers and externally with longitudinal fibers, in-between them being a layer of c oiled fibers that, during the pregnancy, allow the uterus to grow and to be properly irrigated with inventory. The myometrium is crossed by many blood vessels, with predominant venous type structures, amyotrophic lateral sclerosiso know as stratum vasculosum. It was ascertained that the uterine smooth muscular fibers play a major(ip) role in the physiologic haemostasis, namely in closing the vascular structures at this level. (5)During the pregnancy both the uterine body and the cervix become violaceae, due to the strong blood irrigation. The pregnancy is featured by the hypertrophy and the hyperplasia of the myometrium. We whitethorn also notice an increase of the number of elastic and collagen fibers that prevent the uterine argue from breaking. The changes of the cervix are less representative and consist of edema and local congestion. (4) The normal evolution of the pregnancy takes regulate on the grounds of certain fundamental transformations of the endocrine system. Besid es the changes appearing at the level of the genital tract generated by the development of the embryo and of the fetus, the body of the pregnant woman will sustain a stage of more intense hormone activity. (3)The investigations were made on human fresh material taken by the specialty services of obstetrics-gynecology, with the piece of the anatomical pathology service at heart Dr. I. A. Sbarcea Hospital of Brasov and of the specialized study laboratories of the Cellular and Molecular Medicine Department within Carol Davila Medical University of Bucharest.The fragments of the normal non-pregnant uterus were obtained from surgeries such as total and subtotal hysterectomies.The fragments of normal pregnant uterus at due time were taken both from the uterine body and from the cervix.The fragments taken within the specialty services were studied by means of the classical optic microscopy method and by means of the electronic microscopy.The classic histological technique utilise the h ematoxylin- eosin and caravan Gieson staining.The fragments taken for examination by means of the electronic microscope were processed match to the technique that includes as master(prenominal) stages the fixation with glutaraldehyde in cacodyl radical pad for 90 minutes at 4C, washing with cacodyl pad pH 7.4, three times for 5 minutes at 4C incubation in GOMORI environment postfixacion in 0s04 of 1%, for 90 minutes at 4C rinsing in bidistilled water three time for 5 minutes each, at room temperature, block coloring in uranyl acetate for 30 minutes, at 4C in the dark, washing with 10% alcohol, twice, 3 minutes each, at room temperature dehydration with propdenoxide, twice for 5 minutes each at room temperature dehydration with absolute ethylic alcohol, 3 times. 5 minutes each at room temperature dehydration with propilenoxide twice, 5 minutes each at room temperature penetration in Epon with inclusion in propilenoxide (11), for minimum one hour, followed by the evaporation of p ropilenoxide, up to 8 hours, penetration with Epon inclusion for 2 hours, inclusion, ultra sectioning at microtoms, grids contrasting and grids examination at the electronic microscope, obtaining magnifications between lOOOxoOOOx according to Onicescu method, 1998.We monitored the structural and ultra structural changes appearing in the uterine muscular fibers, in the conjugation structures at this level, as well as of the vascular myometrial structures using classical histological and electronic microscopy techniques.Ultra structurally, the uterine muscular fiber presents features common to the smooth muscular fibers of the cavity organs, with a centrally locate, elongated essence.The sarcolemma of the myocytes located at the periphery of the musculus fiber depends to have three layers.The changes appeared at the level of the sarcoplasma may be grouped in two areas.At the level of the central area, the changes of the common organelles are being emphasized, such as the rough e ndoplasmic reticulum made up of several cisterns and vacuoles, of whose membranes ribosomes are being attached at large intervals, free ribosomes and glycogen inclusions, rare flattened sacks and mitochondria presenting a small number of lamella-form christa.At the level of the peripheral area that occupies the larger part, are the special, organelles, forming the contracroofing tile sublayers of the uterine muscular fiber, namely the myofilaments and the dense bodies. The myofilaments, visible at the electronic microscope, are represented only by actin filaments corresponding to the thin filaments of the striated muscle fiber. The myosin filaments are missing, corresponding to the thick filaments of the striated muscle fiber. We noticed ultra structural changes of the uterine muscular fiber, where the myosin appears as dense bodies, noticed as opaque spots, dense at electrons flow, elongated, distributed among the actin filaments. (12)During the pregnancy the myometrium hypertrophi es, process that by its rapidity and amplitude, is a unique phenomenon in the normal adult body, accompanied by spectacular reshuffling of the uterine muscle fiber. In these circumstances, the myocytes grow of about 5-10 times, reaching lengths of 500 micrometers. In the same time, they keep their general shape, but elongations appear extensions especially at the ends. (7)Thus, major changes of the main components of the sarcoplasma are achieved. Both the myofilaments and the dense bodies numerically grow. Unlike the myocyte from the non pregnant uterus, the commune organelles occupy large areas in the sarcoplasma and in the peripheral area of the cubicle. The Golgi complex shows measuring stick and quality changes, becoming one of the most prominent features of the sarcoplasma. The quantity determinations showed an increase of the glycogen content of the pregnant uterine muscle fiber, of approximately 50 times. (5) at a lower place the influence of the ovaries hormones, mixed kin ds of muscle connections appear in the myometrium, peculiarity to the effect of the estrogens or of the progesterone.Under the influence of the estrogen, we may simultaneously notice three types of distinct cell groups, light cells oriented approximately parallel to the more or less smooth surfaces, light cells in transverse arrangement and having inter-located surfaces on wide areas and whorled dark cells, located in transverse arrangement, with wider distances between them. (9)Under the influence of the progesterone, after the ovulation and during pregnancy, we may notice rare light cells, yet, the vacuolated cells dominate, especially after the ovulation. As well, typical spiral cells, with more or less smooth surfaces highlighted.At the level of the intrauterine connective tissue, although the predominant connective cell is the fibroblast, we may also notice macrophages, plasmocytes and masto- cytes, all these cell types being reduced in number. Both the structural appearance and the activity of the connective cells undergo changes that take place under the hormonal influences occurring in the uterine functional cycle. (2)We noticed that in the uterus that prepares for gestation, fibroblasts may undergo metaplasia and may change into myocytes. The pregnancy stresses the most the connective cell, in the sense of accelerating in it the synthesis process of the specific macromolecules, such as the collagen and certain products such as the glycosaminoglycanes. (3)The cervix of the adult woman presents in its structure a large amount of connective tissue, made up of cells, fibres, fundamental substance and a small number of smooth muscle fibres. In the non pregnancy stage, the connective of the cervix seems twice more abundant than the one uterine body. (4)The blood vessels of the myometrium, of artery or vein type, represent the constant and easy element to all myomterial cell metabolic changes, in all the uterine functional phases. This way they insure all myometrial local metabolic activity, of synthesis, on coaction between the myocytes and the surrounding connective, of cell symbiosis changing in pregnancy compared to non pregnancy. (6)On the myometrium fragments of the non pregnantFigure 3 Non pregnant normal uterus. Myometrium.Transmission electron Microscopy x 2950normal uterus and pregnant uterus at due time, by means of the classical histological technique we highlight an arrangement characteristic to a myometrial area where the smooth muscle fibers are placed in fascicles having different directions and sizes. In between them, we may notice weakly represented connective structures, (fig. 1)The image shows a myometrial area where the connective structures are well represented in the non pregnant uterus. In the same time, we may notice that between the fascicules of myometrial muscle fibers, blood vessels of capillary and arterioles type are present in longitudinal section, (fig. 2)Figure 1 Non pregnant normal uterus. Myome trium. Optical Microscopy. Hematoxylin-Eosin stain x 20 development the technique of electronic microscopy, on a microphoto we notice the detail of an area of the sarcoplasma of the uterine smooth muscle fiber. We notice a large number of mitochondria positioned in groups between the myofilaments and especially in a row in the closest neighborhood and along the dense bodies. AmongFigure 4 Normal uterus, pregnant at due time. Myometrium. Optical Microscopy. Hematoxylin-Eosin stain x40the mitochondria there are frequent glycogen inclusions. We may notice the anchoring of the actin filaments of the dense body of the myocyte, (fig. 3)At an magnifying power of 40x, using the classical histological technique, we observe the smooth muscle fibers, with elongated, euchromatic nucleus, with rich sarcoplasma and a cross-sectioned arteriole. The fibroblasts between the myocytes are rare, with dark, rectili almost nucleus, (fig. 4)The figure illustrates myocytes presenting a large, oval- shaped , largely elongated, rich in euchromatine nucleus, with 1-3 nucleoli. The sarcoplasma is abundant. mount the muscle fibers, there is a large amount of connective tissue with fibroblasts and numerous connective fibers, (fig. 5)Figure 2 Non pregnant normal uterus. Myometrium. Optical Microscopy. Van Gieson stain x 20An observed ultrastructural aspect illustrates longitudinally sectioned smooth muscle fibers, with grouped characteristic arrangement, namely the thinned part of a myocyte being near the thicker central area of the neighboring myocytes. Between the muscle fibers there are narrow spaces with less connective matrix. The nucleus of the muscle fibers is predominantly euchromatic and the sarcoplasma near the poles of the nucleus contains cellular organelles, besides them we may observe myofilaments and rare denseodies. The sarcoplasma shows caveolae and dense areas, lear the myocytes we find a fibroblast with large nucleus, ctively euchromatic. Around it, the cytoplasm is loa ded dth cellular organelles, among which we notice the rough ndoplasmic reticulum, as dilated, inter-communicating acks. (fig. 6)Researches based on modern techniques of electronic licroscopy, lead to data related to ultrastructural organization f the uterine smooth muscle fibers, with its particularities in wo distinct functional moments, non pregnancy and regnancy, in healthy women, with uterus having no We observed the vicinity relations between the nyocytes and the connective cells at this level, focusing m the fibroblasts, connective cells considered to be lormone-sensitive, moreover as this type of cells takes art in the composition of organs that are hormone- ensitive such as the uterus or its components. (2)The technique of electronic microscopy highlighted dtrastructural aspects of the smooth muscle fibers, of the connective cells and of the vascular structures of the nyometrium of the non pregnant and pregnant uterus.Studies of electronic microscopy highlighted various tsp ects. Thus, the specific functions of the smooth muscle, uch as tension, contraction and relaxation, are considered to )e the terminus of the coordinated activity of the muscle cells. X/e assumed the existence of a functional syncytium at least or four functional conditions. In the same time, we lescribed the fusion of the neighboring cell membranes, in ertain contact points, namely intercellular junctions type nulla occludens and it may be considered that such onnections would represent the morphological fundamen- als of the functional synctyium. (11) As well, we showed that he number of noticed contact points is small compared to he surface of the cell. According to certain authors, the iifferent forms of cell contacts are granted to various imctional states of the same muscle. The muscle connec- ions thus exist as interdigital cells, on wide surfaces, with anclearly defined cell membranes or as sole, distinct cells.Varied forms of muscle cells seem to help the answer to the ques tion whether the myometrial contraction is isotonic or isometric. From this point of view, we consider that the function of the myometrium resides in an isometric contraction. The observations of other researchers suggest that in the myometrium and in the smooth muscle of other cavity organs, the isometric and isotonic contractions are just different phases of a complex contraction cycle. According to the morphological observations, it seems to be the rhythmic passing from relaxation to isometric contractions and the other way around. (10)According to recent studies, the fine structure of myometrium from pigs collected at well-defined stages during pregnancy was investigated by transmission electron microscopy. The morphology of the pregnant myometrium resembled that in non-pregnant pigs. Thick myofilaments were conspicuous during early pregnancy, unapparent during mid-pregnancy and visible again by days 80-84 and towardsFigure 5 Normal uterus, pregnant at due time. Myometrium. Opt ical Microscopy. Van Gieson stain x20Figure 6 Normal uterus, pregnant at due time. Myometrium. Transmission electron Microscopy xl 500parturition. Gap junctions were extremely rare throughout pregnancy. (8) The researches in the field are ongoing, due to the modern investigation possibilities that, besides the already known investigation methods, mainly refer to the immunohistochemical techniques.